To study the shear performance of segmental prefabricated continuous composite box girder with corrugated steel webs， two scaled test beams were made， including segmental continuous box girder with variable section corrugated steel webs and monolithic continuous box girder with variable section corrugated steel webs in the same size. Through static tests and numerical analyses， the distribution of shear stress and shear ratio of corrugated steel webs are obtained. The results show that the shear stress of the corrugated steel webs of the segmental and monolithic beams at 1/4 of the mid-span is evenly distributed along the beam height， and the shear stress value of the segmental beams is greater than that of the monolithic beams. The calculation formula of shear stress of segmental assembly box girder with variable section corrugated steel webs is derived， and the effect of construction technology on shear stress is considered. The shear ratio of corrugated steel webs of two test beams is less affected by the load and maintains a constant ratio. The shear ratio of the steel web of the two test beams at the position of the middle support is about 50%， and increases along the longitudinal direction of the test beam to both sides. At the position of 1/4 of the middle span， the shear ratio of the steel web of the segment-assembled beam reaches more than 85%， and the shear ratio of the steel web of the whole beam is about 75%. The shear ratio of the two testing beams at the corresponding position of the side span is not much different. The shear strength calculation formula of the AASHTO joint applicable to segmental concrete box girder multiplied by 0.9 can be applied to the calculation of the shear bearing capacity of the joint section. The error between the above formula value and the experimental value as well as with the finite element result is about 5 %. It can better predict the shear strength of steel-concrete composite structure adhesive joints.